四、結(jié)論

在這項(xiàng)工作中，我們研究了 16-E2-16 與 通過(guò)使用紫外-可見(jiàn)光、熒光和 CD 光譜技術(shù)結(jié)合計(jì)算方法來(lái)計(jì)算 Hb。 此外， 該相互作用系統(tǒng)也采用表面張力法。 在 UV-vis 研究的幫助下，我們發(fā)現(xiàn) 16-E2-16 導(dǎo)致血紅素基團(tuán)暴露于水性介質(zhì)。 熒光光譜證實(shí)內(nèi)在猝滅 16-E2-16 的滲透抑制了血紅素對(duì) Trp 的影響 進(jìn)入 Hb 的疏水腔并導(dǎo)致熒光強(qiáng)度增加。 效果還是比較明顯的 只到cmc。 Trp 熒光比 Tyr 受到的影響更大 如同步光譜所示。 表面張力結(jié)果 確定 16-E2-16 的 cmc 值在存在的情況下增加 Hb 以及隨著溫度升高而 cac 保持不變 在所有溫度下保持恒定。 表面張力測(cè)量也 表明膠束化過(guò)程是自發(fā)的、放熱的和 熵驅(qū)動(dòng)。 此外，得出結(jié)論，吸附在 界面優(yōu)于膠束化。 分子建模方法表明 16-E2-16 在 Hb 中的結(jié)合 位于α1和β2鏈中。 它還提供了對(duì)我們的驗(yàn)證 通過(guò)確認(rèn)主要結(jié)合力的實(shí)驗(yàn)結(jié)果 是疏水的和靜電的。 圓二色性表明 16-E2-16 與 Hb 結(jié)合導(dǎo)致 α-螺旋含量減少。 MD 模擬結(jié)果表明 Hb 和 16-E2-16 結(jié)合 Hb 穩(wěn)定在 6000 ps 左右并確認(rèn)構(gòu)象 與 16-E2-16 相互作用后 Hb 發(fā)生了變化，如 我們的 CD 結(jié)果顯示。

致謝

Rajan Patel 博士非常感謝來(lái)自 科學(xué)與工程研究委員會(huì)（SR/S1/PC-19/2011 和 SB/EMEQ-097/2013) 和新德里大學(xué)撥款委員會(huì)， 印度（F.No. 39-841/2010 (SR)）。 Abbul Bashar Khan 博士表示感謝 印度新德里科學(xué)與工程研究委員會(huì)， 提供研究資助 (SB/FT/CS-031/2013)。 作者還 感謝 DST 為 FIST 撥款提供制裁令編號(hào)。 （SR/FIST/LS-541/2012）。

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酯功能化的雙子表面活性劑與血紅蛋白的結(jié)合——摘要、簡(jiǎn)介

酯功能化的雙子表面活性劑與血紅蛋白的結(jié)合——材料和方法

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酯功能化的雙子表面活性劑與血紅蛋白的結(jié)合——結(jié)論、致謝！